angus's picture
angus
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+ 28 Mass Spec Explained~Sleight of hand exposed

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It is time for a little education on mass spectrometry testing, I hope you all find it useful and for those that don’t perhaps you’ll find it interesting. I will keep it as simple as possible without getting too technical.

There are several different types of testing in the mass spectrometry world and we are gonna focus on two for right now. They are the most common results that we see here on this site. They are the HPLC Column test and the Spectrum test. While each one has its purpose and is useful in its own way, without being applied correctly they can be very misleading. At the heart of these tests is the molecular weight of the compound being tested. The spectrum test identifies all the ions within a sample based on their molecular weight within a predefined range. 0-800m/z is the range that all anabolic steroids fall between with the oil, BA, and BB being outside that scope. The molecular weight of the target compound is compared to a known sample to verify the compound is present. Once that is verified we take the quotient of the target compound (X) and the total of unknown ions that make up all the other molecular weights (Y) to arrive at a purity (X/Y). The density of the target compound relative to the suspension is again compared to a known sample to verify if the correct dosage has been made. While that measurement is accurate, it cannot be exact. That is more of a function of the HPLC column test.

The HPLC column test differs from the spectrum test in that it separates all the ions that pass through it and separates them into different groups where it then tests the purity of that individual group. This process is extremely useful for identifying an unknown substance in a compound, as well as it is very precise for showing a dosage. It does not calculate the amount of target compound compared to the other substances in the sample, therefore, the purity calculation that it performs is only on the target compound. (When I say target compound I am referring to the one that we are most interested in such as test or tren.) This purity percentage is very misleading since people think that it is an overall purity, which is not true. To oversimplify it:

Full Spectrum Testing: 10 people in a room, 2 blondes, 8 brunettes = 80% brunettes
HPLC Column testing: 10 People in a room, 2 blondes, 8 brunettes. 2 blondes are put in a different room and now = 100% brunettes.

Now that all that has been defined I get a lot of PM’s from people and sources that are excited because they just got an amazing find from a standup guy in China that wants to send them 99.9% pure raw material and has tests to prove it. When that raw material is put through the spectrum test they are always disappointed in the results, and rightfully so because they were sold on the idea that they had ultra-pure raw material. The problem is that our friends in China are running the HPLC column test, which we know isolates the target compound and then tests it for purity, and are selling it as a full spectrum test. You can spot a column test easily enough because the purity is always 98-99.9% pure. That isn’t to say that 97% isn’t possible with a spectrum test because we have seen that once or twice, but it is very rare.

So the moral of the story is that if you are in the market for raw material and you are being sold a Ferrari at Hyundai price, you might scrutinize the proof a little. Then take your newly gained knowledge and wield it so that you don’t become a victim.

ShreddedCheddar's picture

Waiting on you to ship it to me asshole! Right now I'm just working with what I got!

Engineereddisaster's picture

One doesn't need chloroform. Just take an ether based starting fluid and spay it into a tag that is semi-saturated with mek, (the base for PVC cement.) that works for me in a bind.

ShreddedCheddar's picture

Ill put some padding on them. I don't want them to wake with bruised wrist and sore asses. That would look bad on me.

ShreddedCheddar's picture

Great info! You out some wrinkles in my brain! Learn something everyday! +2!

HllwdBdBoy's picture

So the moral of the story is that if you are in the market for raw material and you are being sold a Ferrari at Hyundai price, you might scrutinize the proof a little.

...and take into consideration you are dealing with China ;)

HailRazor's picture

Excellent explanation. Great analogy. That deserves a +1000

VIKING EVOLUTION's picture

ANGUS u da man but jeez i got a headache right now ....... 8 brunettes is playing on my mind lol

ShreddedCheddar's picture

8 Brunettes and 2 blondes climb in the back of Ed's van..... None come out.

Engineereddisaster's picture

I thought there wAs at least one red head in there?

ShreddedCheddar's picture

That was the blood in her hair when you got done...

Darktide's picture

8 brunettes is playing on my mind lol

I have seen the pictures of all of your women acolytes at your house! Your headache is because 8 brunettes was the low number that you started with years ago and you can't imagine going with such a ridiculous low number again :P!

VIKING EVOLUTION's picture

Hahahaha... youve seen too much in my house already....... next time i must get you drunker then you forget everything lmaoooo

MegaT883's picture

Nice explanation Angus +1

stretch_95's picture

Excellent learning! Thanks!

MegaT883's picture

X2

ANBRO's picture
X3
vhman's picture

Great post and great information. Thanks!

Darktide's picture

That was great angus! Way to dummy it down for us chemical illiterates! I tell you if Eroids primary mission statement is a review site than people like you and beaker are the ones that make factual accurate reviews possible. This is no small contribution on your part thank you for taking the time and using your skills to enrich this community with much needed knowledge!

iFit's picture

Thanks for dumbing that down so I could understand it. Lol. So are you saying that the HPLC is basically worthless for our purposes or does it have value? +2

iFit's picture

Gotcha! Thanks again. This post should be a sticky for sure.