Storage of HCG Before Reconstitution
I remember reading a while ago that HCG should be stored in the fridge before reconstitution and during its use, like all lyopholized powders as in HGH, IGF, HCG, and other peptides. I just looked it up right now and I read mixed messages. Some said to always keep it in the fridge and others said it is fine to keep it at room temp until you reconstitute it. The correct answer I was looking for was printed on the box of the Fertigyn 5000 boxes I have. It states:
For Reconstitution refer package insert.
Storage at 2 C. to 8 C. (35.6 F. to 46.4 F.), protected from light.
Do not freeze.
So according to Sun Pharma, who makes Fertigyn, their HCG should be stored in the fridge before it is reconstituted. While researching I also came across this from http://www.cytoshop.com/ :
Stability: Lyophilized hCG although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution hCG should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. http://www.cytoshop.com/products/hormones/hCG.aspx
They recommend to store their HCH in the freezer until it is reconstituted and then store in the fridge for 2-7 days and to then refreeze if further storage is needed. After seeing this I realized the storage of your HCG depends on what the makers of the product recommend. This should be a reminder for everyone to check their specific makers instructions for proper guidance.
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When it's in powder form I keep it in my safe which is dark and room temperature. Once I add bacteriostatic water I keep the vial refrigerated.
I would be extra careful with this practice. A lot of safes are known to be musky when you open them and at times even moldy (you notice this especially on paper documents). Those conditions are a recipe for problems. FYI.
Here is something I came across about a month ago... it's for IGF, so probably similar for other peptides imo. I'm pasting it because I'm not going to regurgitate it myself... it's good stuff though.
Storage of LR3 IGF-1
*Study conducted by Gropep
The stability of a liquid solution of LR3IGF-I was monitored for a period of two years at storage conditions of -20 C, +4 C, +22 C, and +37 C. The final concentration of LR3IGF-I was in acetic acid. At various time points, samples were taken and compared to a lyophilized control (stored at 4 C). Listed below are the stability results for each respective storage condition.
Storage Condition: -20 C (-4 F)
Biological Potency No Change up to 2 years
Immunological Activity No Change up to 2 years
Mobility of Protein No Change up to 2 years
Elution Profile by reversed phased HPLC No Change up to 2 years
Storage Condition: +4 C (39.2 F)
Biological Potency No Change up to 2 years
Immunological Activity No Change up to 2 years
Mobility of Protein No Change up to 2 years
Elution Profile by reversed phased HPLC No Change up to 2 years
Storage Condition: +22 C (71.6 F)
Biological Potency No Change up to 2 years
Immunological Activity No Change up to 2 years
Mobility of Protein No Change up to 2 years
Elution Profile by reversed phased HPLC No Change up to 2 years
Storage Condition: +37 C (98.6 F)
Biological Potency No Change up to 1 year
Immunological Activity No Change up to 1 year
Mobility of Protein No Change up to 1 year
Elution Profile by reversed phased HPLC No Change up to 1 year
In conclusion, there is no significant difference in the potency of LR3IGF-I associated with the storage of the liquid formulation when stored at this range of temperatures. There is no evidence for loss of biological activity at any of the tested temperatures when stored as a liquid product. As you can see IGF can be quite stable for even a year at room temperature, but if you want to keep it around for a while then stick it into the fridge. So in my opinion the best way to store LR3 that is suspended in BA (benzoic acid) is in the freezer. The BA won’t allow it to freeze. And if you have it suspended in AA (acetic acid), store it in the fridge.
Thanks for posting that, that is some great info.
Here's what I know about hCG. Although I don't doubt the validity of what you are posting it does go against common practices in the industry. Remember legitimate hCG is moved around by the postal system where it is not refrigerated. It's kept in pharmacies and warehouses unrefrigerated for months on end and in the end it works just fine.
I have a lot of experience with hCG and here are some facts that I know.
You can keep it unreconstituted in a cool dry place away from direct light for a long time past expiration (it does lose potency with time).
You can even keep it for weeks unrefrigerated after it's been reconstituted and the only thing that happens is you lose a small amount of potency ( I know I will probably catch a lot of flak for this statement).
hCG is a fragile and can be damaged during the mixing process. You can't just squirt BAC water at it under high pressure.
Hope this helps.
When you consider the amount of huge variations in humidity, and pressures, and handling that these products go through.. SURELY they are almost indestructible? I mean, from point of distribution from the OEM, it must go through a myriad of changes...
My view? Keep it cool, dry and out of direct sunlight.
;-)
Actually hCG is pretty fragile in some regards. If you squirt the BAC water at it too hard believe it or not you can damage the compound.
So we're told. Also, told to roll gently and never shake, etc... My point was, is it really this environmentally sensitive?
As far as being environmentally sensitive as long as you stay within normal parameters you are good to go.
and you are correct not to shake it.
Never shook it bro'. Though, once the enzyme is active, when a soluble is added, then, agitation is not recommended, though... WHY? The trauma suffered from separation from the original vessel, and the introduction to subcutaneous must be huge trauma... So, my point, is this is a lot tougher than we are told..
Look at the "red button, do not press" test? Everyone presses it eventually, so, the OEM's are being safe. is all...
Anyway, I tend to eddy on the side of caution with EVERYTHING I use...
I just sent an Email to the seller of my generic HCG, asking for his recommended storage method. It doesn't have any directions on their website or on the vial itself, but until I hear back from them I will keep it in my fridge, like the Fertigyn.
http://www.eroids.com/pics/hcg-from-mtp