8ius before bed, nightly, is what I do.

There really isn’t a single “best” HGH protocol. It depends on the goal (general health, recovery, lean gain, or contest prep). Some common approaches you’ll see discussed are:
2–4 IU/day: Probably the most common range for recreational and amateur bodybuilders. Many use it consistently for body composition and recovery. 4–6 IU/day: More common among advanced competitors, but the likelihood of side effects (fluid retention, numbness/tingling, insulin resistance, joint pain) increases. 6+ IU/day: Generally reserved for elite competitors. Side effects become much more common, and more is not necessarily better.

Some people split the daily amount into two injections (morning and later in the day), while others take it all at once. There isn’t strong evidence that one method is clearly superior for muscle growth.

Reconstitution: use bacteriostatic water because it helps preserve sterility for multiple doses. Add the diluent slowly down the inside of the vial rather than spraying directly onto the powder, gently swirl (don’t shake vigorously), refrigerate after mixing. Amount of BAC depends on the amount of HGH in the vial. 10, 20, 40? And how much you want for each injection. 10 IU with 1 ML of BAC will give you 5 shots at 2 IU per injection.

Whatever protocol someone chooses, it’s worth keeping an eye on fasting glucose, HbA1c, lipids, blood pressure, and IGF-1, since HGH can affect insulin sensitivity and other metabolic markers over time.