Sciroxx's picture
Sciroxx
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+ 6 Some important info on GH testing

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I would like to bring to your attention few facts is relevance to testing and evaluating GH product.

Most, if not all tests which are posted here on GH products are serum GH and IGF1 tests.

Serum GH test indicates on the present and quantity of GH in the product. It's certainly not an accurate indication to its clinical effect. It's a good start as it confirms that what the label declares is there, but it's certainly not the whole picture -

  • GH is a very delicate molecule. Slight harm in its 3 dimentional folding may harm its biological effect. For instrance the the minor change in the slightest angle of the binding are to the receptor may prevent activiation of the receptor. So even if in seum test the GH is as high as it should it's still doesn't guarantee it's full clinical effect

  • When extracting proteins such as GH they have a tendacy to create isomers - I really hope I use the right term in English - what I refer to is that 2 GH molecules or more are folded together, this form of the GH is biologically not active. Serum tests clearly doesn't identify in what form the GH is

So even if a a serum test show proper presence and quantity of GH colecules it certainly don't indicate on its effect in vivo.

The USP defines certain protocol to asses the biological effect of GH. In a nutshell it tests the effect of GH which is injected by certain protocol to mice, which their hypophiza is removed, and check along 3-6 months their liner growth (and other parameters). This is quite complicated clinical trial.

In our facility we use more advanced biological assay tests - we check the prolifiration of human cells under direct exposure to the GH in the product. this is currently the ultimate test, as it test directly the interaction of the GH with target receptor and quantifies its direct clinical effect.
When mice are injected with GH there are more factors are involved in their growth, like nutrition, genetic parameters, enviorment influence etc', of course the test tries to isolate the effect of GH by giving similar nutrition etc' to the mice, but as you understand it can't eliminate 100% the influence of such conditions

This brings us to the IGF1 serum tests. IGF1 serum tests clearly gives a valid indication to the clinical effect of the GH, but the results may widely vary as the IGF secretion (mainly in liver, as well as kidneys) depends also on many other factors such as nutrition, other hormonees like sex hormones, stress hormones, insulin and glucagon, so the numbers this test brings are not an ultimate measure
I really hope that I use the right terms in English above, I'll post a summery from professor on Wednesday, including specified biological assay tests and graphs, as well as SDS-HPLC tests along the extraction and purification process of our product which confirms it's 98+% monomer is active pure form

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ScorchedEarth's picture

Good read +1

growthman's picture

Great post! I was looking for this exact info a few days ago; Quantity vs Quality. Thanks for sharing this, sir.